Isothermal amplification PCR in 30 minutes with no RNA extraction

Let's return to normal

ender MASS is an in-vitro diagnostic test kit based on a rapid molecular isothermal nucleic acid amplification technology.

ender MASS is reliably detecting the mutants from UK (20I/501Y.V1, B.1.1.7), South Africa (20H/501Y.V2, B.1.351) and Brazil (20J/501Y.V3, P.1).

It is intended for the qualitative detection of nucleic acid ORF-1a gene sequence from the SARS-CoV-2 Viral RNA in direct nasal, nasopharyngeal or throat swabs and saliva from individuals suspected of COVID-19.

The test is performed on standard real-time Polymerase Chain Reaction (RT-PCR) cyclers.

The ender MASS test kit is intended for use by medical professionals or trained operators who are proficient in using RT-PCR cyclers and performing standard molecular biological laboratory procedures.

ender MASS is the optimal laboratory-based solution for mass testing in a public health setup, where there is a need for identifying all infectious individuals with a sufficiently high virus load to pose a threat to others.

The ender MASS test does not require a conventional RNA extraction, saving hours of laboratory time, reagents and decreasing the use of consumables.

Rapid, Reliable and
Cost-Effective

Rapid

5 minutes sample preparation as no RNA extraction is needed & 30 minutes isothermal amplification time.

Reliable

Performance at highest industry level.

Cost-Effective

Significantly increase your throughput with the same infrastructure and staff.

Basic steps & Workflow

Approx. 5 minutes sample preparation*
*Depending on sample throughput & automation setup
1
no RNA extraction required
2
30 minutes isothermal amplification time
3
Approx. 5 minutes melting curve analysis**
** 5 – 20 minutes depending on PCR cycler
4
Specimen collection
(not provided with the kit)​
1
Thermolysis of sample
(reagents provided with the kit)​
2
Isothermal amplification
(reagents provided in kit)​
3
Data Analysis
(according to PCR cycler used)
4

Your benefits using ender MASS

Performance Data

Sensitivity 93%
Specificity 100%
PPV 100%
NPV 99.2%
(CI 97.7%-99.7%) at an estimated prevalence of 10%
LOD of 0 c/µl
or 480 copies per reaction

ender MASS applied to your needs

Provide periodical tests:

The solution for:

Get your ender MASS solution here