Technology

The ender Technology

The ender diagnostics COVID-19 assays are in-vitro diagnostic (IVD) test kits based on a proprietary rapid molecular isothermal nucleic acid amplification technology method. They are intended for the qualitative detection of nucleic acid ORF-1a gene sequence from the SARS-CoV-2 Viral RNA in direct nasal, nasopharyngeal or throat swabs and saliva from individuals suspected of COVID-19.

Test Principles

The ender products are based on an isothermal amplification and real-time detection technology of the SARS-CoV-2 ORF-1a gene sequence. The amplification reaction takes place at a constant temperature of 65 °C for 30 min.

No thermal cycling is required since the polymerase harbors a helicase activity. Thus, primer binding and amplification happen instantaneously and continuously. A separate reverse transcription step is not required due to reverse transcription activity of the enzyme used. Reverse transcribed and amplified DNA is detected by a fluorescent DNA-intercalating dye. After isothermal amplification, a melting curve analysis is performed to distinguish the SARS-CoV-2 amplicon from the included internal amplification control (IC). The two targets are identified based on their melting temperature.

Sample Preparation

Works with a simple 3 minutes thermolysis prior to amplification / real-time detection.
Uses RNA extraction by a commercially available extraction kit or an automated extraction system.

Workflow

5 minute sample preparation only
1
No RNA extraction required
2
30 minutes for isothermal amplification PCR
3
5 minutes melting curve analysis
4
Time required for RNA extraction depending on the methodology and number of samples
1
30 minutes for isothermal amplification PCR
2
5 minutes melting curve analysis
3

Isothermal Amplification

Our isothermal amplification PCR enables a reliable identification of infectious SARS-COV-2 carriers and an increase in testing capacity.

If you want to read more about our solution for your demand in test capacity for screening, download this overview paper:
Infected vs. Infectious and testing solutions.

Benefits

We provide a novel approach for the effective management of the COVID-19 pandemic with patented technology.

Shorter time to result

Uncompromised sensitivity and specificity

Higher sample throughput

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Get your ender MASS solution here

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enderMASS-80
80 reactions
enderMASS-240
240 reactions
enderMASS-400
400 reactions

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Get your ender LAB solution here

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enderLAB-50
50 reactions
enderLAB-100
100 reactions
enderLAB-50
500 reactions

Reservation Form

If you are interested in ender MOBILE and want to secure your volume for market entry please register in the reservation form.

This form helps us keep you informed about the progress of ender MOBILE as well as understand the market demand for our launch process.

Your data will be kept in our customer database for product reservations.

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We will send you further information about the development of ender MOBILE.