The ender products are based on an isothermal amplification and real-time detection technology of the SARS-CoV-2 ORF-1a gene sequence. The amplification reaction takes place at a constant temperature of 65 °C for 30 min.
No thermal cycling is required since the polymerase harbors a helicase activity. Thus, primer binding and amplification happen instantaneously and continuously. A separate reverse transcription step is not required due to reverse transcription activity of the enzyme used. Reverse transcribed and amplified DNA is detected by a fluorescent DNA-intercalating dye. After isothermal amplification, a melting curve analysis is performed to distinguish the SARS-CoV-2 amplicon from the included internal amplification control (IC). The two targets are identified based on their melting temperature.
Our isothermal amplification PCR enables a reliable identification of infectious SARS-COV-2 carriers and an increase in testing capacity.
We provide a novel approach for the effective management of the COVID-19 pandemic with patented technology.
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