The emma PCR (ender molecular multiplex approach) is an innovative diagnostic test designed to rapidly identify and differentiate mastitis pathogens in dairy cows using PCR technology. It is developed by ender diagnostics in Switzerland and can be used directly in veterinary practices.

emma PCR uses real-time PCR technology to detect specific mastitis pathogens within 90 minutes. The process involves sample preparation, PCR amplification, and automated analysis, all conducted in a veterinary practice setting.

emma PCR is capable of detecting a wide range of pathogens, including:

• Contagious Panel:

  • Staphylococcus spp.
  • Staphylococcus aureus
  • Streptococcus spp.
  • Streptococcus uberis
  • Streptococcus agalactiae
  • Mycoplasma spp.
  • E. coli
  • Internal control
    
    

• Environmental Panel:

  • Staphylococcus spp.
  • Staphylococcus aureus
  • Streptococcus spp.
  • Streptococcus uberis
  • Enterococcus spp.
  • Klebsiella spp.
  • E. coli
  • Internal control

• Rapid Results: Provides results in just 90 minutes.
• Accurate Diagnostics: Enables evidence-based treatment decisions.
• Reduced Antibiotic Use: Helps reduce unnecessary antibiotic use by up to 40%.
• Economic Advantage: Generates additional revenue for veterinary practices by allowing direct billing for tests.
• User-Friendly: Requires minimal training and no specialized laboratory skills.

By providing rapid and precise pathogen identification, emma PCR allows veterinarians to make informed treatment decisions, selecting appropriate antibiotics or opting out of antibiotic use when unnecessary. This targeted approach improves treatment outcomes and reduces the risk of antibiotic resistance.

The test involves the following steps:

1. Sample Collection: Collect a milk sample from the cow.
2. Sample Preparation: Use the provided lysis tube and homogenize the sample.
3. PCR Amplification: Run the PCR process with the selected panel (contagious or environmental).
4. Automated Analysis: Results are automatically analyzed through a web-based system.

emma PCR is designed to be user-friendly and can be easily learned with minimal training. Ender diagnostics provides comprehensive training to ensure veterinarians and staff can efficiently conduct the test.

Speed: emma PCR provides results in 90 minutes, whereas traditional microbiology can take up to 72 hours.
Pathogen Detection: emma PCR detects a broader range of pathogens, including Mycoplasma spp., which are often missed by traditional methods.
No Culture Required: emma PCR does not require pathogen culture, reducing safety risks and time delays.

No, emma PCR does not detect antibiotic resistance or resistance genes. It focuses on rapid pathogen identification to facilitate evidence-based treatment. For resistance monitoring, regular microbiological testing is recommended.

By conducting tests in-house, veterinary practices can generate additional revenue through direct billing. The rapid turnaround time also enhances service quality and client satisfaction.

Yes, emma PCR is cost-effective due to its rapid results, reduced antibiotic usage, and ability to keep testing revenue within the practice. It offers a competitive advantage over slower, traditional testing methods.

Ender diagnostics offers comprehensive training and support, including installation guidance, user training, and technical assistance to ensure seamless integration into veterinary practices.

Yes, emma PCR is designed for use in various veterinary settings, from large animal practices to smaller clinics, without requiring specialized laboratory infrastructure.

The emma qPCR system requires approximately 15 minutes of hands-on time for preparation of 8 samples. The rest of the analysis is automated, with pathogen identification being completed within 55 minutes through a web-based evaluation​​.

For more information, demonstrations, or to request training, please contact ender diagnostics at:

• Email: emma-service@enderdiagnostics.com

Or simply set a meeting with us: